Anti-MICALL2 Antibody Picoband®, Dylight550 Conjugate

Boster Bio Anti-MICALL2 Antibody Picoband® catalog # A12852-1. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Enables filamin binding activity. Involved in positive regulation of protein targeting to mitochondrion. Predicted to be located in several cellular components, including bicellular tight junction; neuron projection; and recycling endosome. Predicted to colocalize with stress fiber.

Synonyms

Mediator of RNA polymerase II transcription subunit 9; Mediator complex subunit 9; MED9; MED25;

Gene Name

mediator complex subunit 9

Gene ID

79778

UniProt

Q8IY33

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MICALL2 recombinant protein (Position: K132-Q741).

Clonality

Polyclonal

Tissue Specificity

Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Sakane, A., Yano, T. A., Uchihashi, T., Horikawa, K., Hara, Y., Imoto, I., ... & Sasaki, T. (2021). JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form tubular recycling endosomes. Communications biology, 4(1), 551. 2. Zhu, L. Y., Zhang, W. M., Yang, X. M., Cui, L., Li, J., Zhang, Y. L., ... & Zhang, Z. G. (2015). Silencing of MICAL-L2 suppresses malignancy of ovarian cancer by inducing mesenchymal–epithelial transition. Cancer letters, 363(1), 71-82. 3. Min, P., Zhao, S., Liu, L., Zhang, Y., Ma, Y., Zhao, X., ... & Du, J. (2019). MICAL‐L2 potentiates Cdc42‐dependent EGFR stability and promotes gastric cancer cell migration. Journal of cellular and molecular medicine, 23(6), 4475-4488.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

109 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -