Anti-PIGS Antibody Picoband®, Dylight550 Conjugate

Boster Bio Anti-PIGS Antibody Picoband® catalog # A06942-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

GPI transamidase component PIG-S is an enzyme that in humans is encoded by the PIGS gene. This gene encodes a protein that is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.

Synonyms

Semaphorin-3E; SEMA3E; KIAA0331; SEMAH

Gene Name

semaphorin 3E

Gene ID

94005

UniProt

Q96S52

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PIGS recombinant protein (Position: R184-D504).

Clonality

Polyclonal

Tissue Specificity

Detected in the sinusoidal epithelium in the red pulp of spleen. Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

Applications

ELISA,Flow Cytometry,IF,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Efthymiou, S., Dutra-Clarke, M., Maroofian, R., Kaiyrzhanov, R., Scala, M., Alvi, J. R., Sultan, T., Christoforou, M., Nguyen, T. T. M., Mankad, K., Vona, B., Rad, A., Striano, P., Salpietro, V., Guillen Sacoto, M. J., Zaki, M. S., Gleeson, J. G., Campeau, P. M., Russell, B. E., Houlden, H. Expanding the phenotype of PIGS-associated early onset epileptic developmental encephalopathy. Epilepsia 62: e35-e41, 2021. 2. Gross, M. B. Personal Communication. Baltimore, Md. 7/21/2020. 3. Nguyen, T. T. M., Murakami, Y., Wigby, K. M., Baratang, N. V., Rousseau, J., St-Denis, A., Rosenfeld, J. A., Laniewski, S. C., Jones, J., Iglesias, A. D., Jones, M. C., Masser-Frye, D., and 7 others. Mutations in PIGS, encoding a GPI transamidase, cause a neurological syndrome ranging from fetal akinesia to epileptic encephalopathy. Am. J. Hum. Genet. 103: 602-611, 2018.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

87148 MW

Observed Molecular Weight

65 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -