Anti-OMP Antibody Picoband®, Dylight550 Conjugate

Boster Bio Anti-OMP Antibody Picoband® catalog # A01781-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Olfactory marker protein is uniquely associated with the mature olfactory receptor neurons in many vertebrate species from fish to man. The OMP gene structure and protein sequence are highly conserved between mouse, rat and human. Results of the mouse knockout studies show that OMP-null mice are compromised in their ability to respond to odor stimuli, and that OMP represents a novel modulatory component of the odor detection/signal transduction cascade.

Synonyms

Importin subunit alpha-1;Karyopherin subunit alpha-2;RAG cohort protein 1;SRP1-alpha;KPNA2;RCH1, SRP1;

Gene Name

Importin subunit alpha-1

Gene ID

4975

UniProt

P47874

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human OMP recombinant protein (Position: E43-L163).

Clonality

Polyclonal

Tissue Specificity

Expressed ubiquitously.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and ly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The ionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Buiakova, O. I., Baker, H., Scott, J. W., Farbman, A., Kream, R., Grillo, M., Franzen, L., Richman, M., Davis, L. M., Abbondanzo, S., Stewart, C. L., Margolis, F. L. Olfactory marker protein(OMP) gene deletion cause altered physiological activity of olfactory sensory neurons. Proc. Nat. Acad. Sci. 93: 9858-9863, 1996. 2. Evans, K. L., Fantes, J., Simpson, C., Arveiler, B., Muir, W., Fletcher, J., van Heyningen, V., Steel, K. P., Brown, K. A., Brown, S. D. M., St. Clair, D., Porteous, D. J. Human olfactory markerprotein maps close to tyrosinase and is a candidate gene for Usher syndrome type I. Hum. Molec. Genet. 2: 115-118, 1993. 3. Rogers, K. E., Dasgupta, P., Gubler, U., Grillo, M., Khew-Goodall, Y. S., Margolis, F. L.Molecular cloning and sequencing of a cDNA for olfactory marker protein. Proc. Nat. Acad. Sci. 84: 1704-1708, 1987.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

57862 MW

Observed Molecular Weight

17 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -