Anti-TPPP Antibody Picoband®, Dylight550 Conjugate

Boster Bio Anti-TPPP Antibody Picoband® catalog # A04389-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Tubulin polymerization-promoting protein is a protein that in humans is encoded by the TPPP gene. Enables several functions, including GTPase activity; magnesium ion binding activity; and protein homodimerization activity. Involved in several processes, including microtubule cytoskeleton organization; negative regulation of tubulin deacetylation; and positive regulation of protein polymerization. Located in several cellular components, including mitochondrion; mitotic spindle; and perinuclear region of cytoplasm. Colocalizes with microtubule and microtubule bundle.

Synonyms

"Long-chain-fatty-acid--CoA ligase 4; Long-chain acyl-CoA synthetase 4; LACS 4; ACSL4; ACS4, FACL4, LACS4 "

Gene Name

acyl-CoA synthetase long chain family member 4

Gene ID

11076

UniProt

O94811

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TPPP recombinant protein (Position: M1-K219).

Clonality

Polyclonal

Tissue Specificity

Expressed in the prostate gland and prostate cancers.

Applications

ELISA,Flow Cytometry,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:24269233, PubMed:22633490, PubMed:21242590). Preferentially activates arachidonate and eicosapentaenoate as substrates (PubMed:21242590). Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion (PubMed:21242590).

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Martin, C. P., Vazquez, J., Avila, J., Moreno, F. J. P24, a glycogen synthase kinase 3 (GSK 3) inhibitor. Biochim. Biophys. Acta 1586: 113-122, 2002. 2. Seki, N., Hattori, A., Sugano, S., Suzuki, Y., Nakagawara, A., Muramatsu, M., Hori, T., Saito, T. A novel human gene whose product shares significant homology with the bovine brain-specific protein p25 on chromosome 5p15.3. J. Hum. Genet. 44: 121-122, 1999. 3. Tirian, L., Hlavanda, E., Olah, J., Horvath, I., Orosz, F., Szabo, B., Kovacs, J., Szabad, J., Ovadi, J. TPPP/p25 promotes tubulin assemblies and blocks mitotic spindle formation. Proc. Nat. Acad. Sci. 100: 13976-13981, 2003.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.