Anti-CENPF Antibody Picoband®, Dylight550 Conjugate

Boster Bio Anti-CENPF Antibody Picoband® catalog # A03311-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Centromere protein F is a protein that in humans is encoded by the CENPF gene. This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.

Synonyms

Glutathione S-transferase Mu 3; GST class-mu 3; GSTM3-3; hGSTM3-3; GSTM3; GST5;

Gene Name

glutathione S-transferase mu 3

Gene ID

1063

UniProt

P49454

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CENPF recombinant protein (Position: Q129-R3078).

Clonality

Polyclonal

Tissue Specificity

Testis and brain.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Filges, I., Bruder, E., Brandal, K., Meier, S., Undlien, D. E., Waage, T. R., Hoesli, I., Schubach, M., de Beer, T., Sheng, Y., Hoeller, S., Schulzke, S., Rosby, O., Miny, P., Tercanli, S., Oppedal, T., Meyer, P., Selmer, K. K., Stromme, P. Stromme syndrome is a ciliary disorder caused by mutations in CENPF. Hum. Mutat. 37: 359-363, 2016. Note: Erratum: Hum. Mutat. 37: 711 only, 2016. 2. Fowler, K. J., Saffery, R., Irvine, D. V., Trowell, H. E., Choo, K. H. A. Mouse centromere protein F (Cenpf) gene maps to the distal region of chromosome 1 by interspecific backcross analysis. Cytogenet. Cell Genet. 82: 180-181, 1998. 3. Gross, M. B. Personal Communication. Baltimore, Md. 8/24/2016.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

358 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -