Anti-TIMM29 Antibody Picoband®, Dylight550 Conjugate

Boster Bio Anti-TIMM29 Antibody Picoband® catalog # A31934. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as the external driving force. Required for the stability of the TIM22 complex and functions in the assembly of the TIMM22 protein into the TIM22 complex. May facilitate cooperation between TIM22 and TOM complexes by interacting with TOMM40.

Synonyms

Protein Bop; BH3-only protein; Retrotransposon Gag-like protein 10; RTL10; BOP, C22orf29

Gene Name

retrotransposon Gag like 10

Gene ID

90580

UniProt

Q9BSF4

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TIMM29 recombinant protein (Position: A37-R260).

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Could induce apoptosis in a BH3 domain-dependent manner. The interaction network of Bcl-2 family members may play a key role in modulation RTL10/BOP intrinsic apoptotic signaling activity.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Callegari, S., Richter, F., Chojnacka, K., Jans, D. C., Lorenzi, I., Pacheu-Grau, D., Jakobs, S., Lenz, C., Urlaub, H., Dudek, J., Chacinska, A., Rehling, P. TIM29 is a subunit of the human carrier translocase required for protein transport. FEBS Lett. 590: 4147-4158, 2016. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 02/28/2017. 3. Kang, Y., Baker, M. J., Liem, M., Louber, J., McKenzie, M., Atukorala, I., Ang, C.-S., Keerthikumar, S., Mathivanan, S., Stojanovski, D. Tim29 is a novel subunit of the human TIM22 translocase and is involved in complex assembly and stability. eLife 5: e17463, 2016. Note: Electronic Article.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

14377 MW

Observed Molecular Weight

29 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -