Anti-GRSF1 Antibody Picoband®, Dylight550 Conjugate

Boster Bio Anti-GRSF1 Antibody Picoband® catalog # A07576-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

G-rich sequence factor 1 (GRSF1) is a regulator of post-transcriptional mitochondrial gene expression. The protein encoded by this gene is a cellular protein that binds RNAs containing the G-rich element. The protein is localized in the cytoplasm, and has been shown to stimulate translation of viral mRNAs in vitro. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

C-C motif chemokine 16; Chemokine CC-4; HCC-4; Chemokine LEC; IL-10-inducible chemokine; LCC-1; Liver-expressed chemokine; Lymphocyte and monocyte chemoattractant; LMC; Monotactin-1; MTN-1; NCC-4; Small-inducible cytokine A16; CCL16; ILINCK; NCC4; SCYA16

Gene Name

C-C motif chemokine ligand 16

Gene ID

2926

UniProt

Q12849

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human GRSF1 recombinant protein (Position: Q119-M405).

Clonality

Polyclonal

Tissue Specificity

Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

References & Citations

1. Banga, S. S., Ozer, H. L., Wilusz, J. Assignment of GRSF1 encoding a poly(A)+ mRNA binding protein to human chromosome 4q13. Cytogenet. Cell Genet. 73: 295-296, 1996. 2. Qian, Z., Wilusz, J. GRSF-1: a poly(A)+ mRNA binding protein which interacts with a conserved G-rich element. Nucleic Acids Res. 22: 2334-2343, 1994. 3. Qian, Z., Wilusz, J. An RNA-binding protein specifically interacts with a functionally important domain of the downstream element of the simian virus 40 late polyadenylation signal. Molec. Cell. Biol. 11: 5312-5320, 1991.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

157578 MW

Observed Molecular Weight

53 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -