Anti-Cyclophilin E/PPIE Antibody Picoband®, Dylight550 Conjugate

Boster Bio Anti-Cyclophilin E/PPIE Antibody Picoband® catalog # A08021-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an enzyme which in humans is encoded by the PPIE gene on chromosome 1. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.

Synonyms

BMP and activin membrane-bound inhibitor homolog; Non-metastatic gene A protein; Putative transmembrane protein; NMA; BAMBI; NMA

Gene Name

BMP and activin membrane bound inhibitor

Gene ID

10450

UniProt

Q9UNP9

Host

Rabbit

Reactivity

Human,Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Cyclophilin E/PPIE recombinant protein (Position: M1-V301).

Clonality

Polyclonal

Tissue Specificity

Expressed in adult liver.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Negatively regulates TGF-beta signaling.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Kim, J.-O., Nau, M. M., Allikian, K. A., Makela, T. P., Alitalo, K., Johnson, B. E., Kelley, M. J. Co-amplification of a novel cyclophilin-like gene (PPIE) with L-myc in small cell lung cancer cell lines. Oncogene 17: 1019-1026, 1998. 2. Mi, H., Kops, O., Zimmermann, E., Jaschke, A., Tropschug, M. A nuclear RNA-binding cyclophilin in human T cells. FEBS Lett. 398: 201-205, 1996.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

17136 MW

Observed Molecular Weight

35 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Mouse Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -