Anti-VMAT1/SLC18A1 Antibody Picoband®, Dylight550 Conjugate

Boster Bio Anti-VMAT1/SLC18A1 Antibody Picoband® catalog # A07692-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Chromaffin granule amine transporter is a protein that in humans is encoded by the SLC18A1 gene. This gene is mapped to 8p21.3. The vesicular monoamine transporter acts to accumulate cytosolic monoamines into vesicles, using the proton gradient maintained across the vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine.

Synonyms

Chromaffin granule amine transporter; Solute carrier family 18 member 1; Vesicular amine transporter 1; VAT1; SLC18A1; VAT1; VMAT1

Gene Name

solute carrier family 18 member A1

Gene ID

6570

UniProt

P54219

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human VMAT1/SLC18A1 recombinant protein (Position: M1-E510).

Clonality

Polyclonal

Tissue Specificity

Highly expressed in chromaffin cells of the adrenal medulla (at protein level). Detected in peripheral sympathetic ganglia (at protein level). Found in some paracrine cells in stomach and duodenum (at protein level).

Applications

ELISA,Flow Cytometry,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles of neuroendocrine and endocrine cells.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Liu, Y., Peter, D., Roghani, A., Schuldiner, S., Prive, G. G., Eisenberg, D., Brecha, N., Edwards, R. H. A cDNA that suppresses MPP+ toxicity encodes a vesicular amine transporter. Cell 70: 539-551, 1992. 2. Peter, D., Finn, J. P., Klisak, I., Liu, Y., Kojis, T., Heinzmann, C., Roghani, A., Sparkes, R. S., Edwards, R. H. Chromosomal localization of the human vesicular amine transporter genes. Genomics 18: 720-723, 1993. 3. Roghani, A., Welch, C., Xia, Y.-R., Liu, Y., Peter, D., Finn, J. P., Edwards, R. H., Lusis, A. J. Assignment of the mouse vesicular monoamine transporter genes, Slc18a1 and Slc18a2, to chromosomes 8 and 19 by linkage analysis. Mammalian Genome 7: 393-394, 1996.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

49229 MW

Observed Molecular Weight

56 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.25-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -

Subcellular Location

Cytoplasmic vesicle membrane. Multi-pass membrane protein. Synaptic vesicle membrane. Multi-pass membrane protein. Endoplasmic reticulum membrane. Multi-pass membrane protein.