Anti-RAB11B Antibody Picoband® (monoclonal, 6C5) (Dylight488 Conjugate)

Boster Bio Anti-RAB11B Antibody Picoband® (monoclonal, 6C5) catalog # M04526. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Ras-related protein Rab-11B is a protein that in humans is encoded by the RAB11B gene. It is mapped to 19p13.2. The Ras superfamily of small GTP-binding proteins, which includes the Ras, Ral, Rho, Rap, and Rab families, is involved in controlling a diverse set of essential cellular functions. The Rab family, including RAB11B, appears to play a critical role in regulating exocytotic and endocytotic pathways.

Synonyms

Ras-related protein Rab-11B;GTP-binding protein YPT3; RAB11B;YPT3

Gene Name

RAB11B,member RAS oncogene family

UniProt

Q15907

Host

Mouse

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human RAB11B, which shares 97.4% and 100% amino acid (aa) sequence identity with mouse and rat RAB11B, respectively.

Clonality

Monoclonal

Clone

Clone: 6C5

Tissue Specificity

Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.

Applications

Flow Cytometry,IF,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking,from the formation of transport vesicles to their fusion with membranes.Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors ly responsible for vesicle formation,movement,tethering and fusion.That Rab plays a role in endocytic recycling,regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR,epithelial sodium channel/ENaC,potassium voltage- gated channel,and voltage-dependent L-type calcium channel.May also regulate constitutive and regulated secretion,like insulin granule exocytosis.Required for melanosome transport and release from melanocytes.Also regulates V-ATPase intracellular transport in response to extracellular acidosis.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Mouse IgG2b

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Subcellular Location

Recycling endosome membrane;Lipid-anchor;Cytoplasmic side.Cytoplasmic vesicle,secretory vesicle,synaptic vesicle membrane;Lipid-anchor;Cytoplasmic side.Cytoplasmic vesicle,phagosome membrane;Lipid-anchor;Cytoplasmic side.Recruited to phagosomes containing S.aureus.