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Anti-Cytokeratin 20/Krt20 Antibody Picoband® (Dylight488 Conjugate)

Selected Variant

A02828-2-Dylight488

Size: 100 µg

Boster Bio Anti-Cytokeratin 20/Krt20 Antibody Picoband® catalog # A02828-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Keratin 20, often abbreviated CK20, is a protein that in humans is encoded by the KRT20 gene. This gene encodes a member of the keratin protein family and is found within a cluster of cytokeratin genes on chromosome 11. Keratins are cytoskeletal proteins that are preferentially expressed in epithelial cells. The encoded protein may help maintain intermediate filament organization in intestinal epithelium. Phosphorylation of this protein may also influence mucin secretion in the small intestine.

Synonyms

Keratin, type I cytoskeletal 20; Cytokeratin-20; CK-20; Keratin-20; K20; Krt20

Gene Name

keratin 20

UniProt

Q9D312

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse Cytokeratin 20/Krt20 recombinant protein (Position: R29-V431).

Clonality

Polyclonal

Tissue Specificity

Expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Also expressed in epithelial cells of bladder, ileum and stomach and at lower levels in pancreas and earskin. The phosphorylated form is nearly exclusively expressed in goblet cells of the small intestine and in the lumen-proximal cells of the colon (at protein level). Also expressed in jejunum and duodenum.

Applications

ELISA,Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25μg/ml, Human, Mouse<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, Rat<br> Flow Cytometry (Fixed)|1-3μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5μg/ml, -<br>

Subcellular Location

Cytoplasm.

Protein Name

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

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