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Anti-Alpha Internexin/INA Antibody Picoband® (Dylight488 Conjugate)

Boster Bio Anti-Alpha Internexin/INA Antibody Picoband® catalog # PB10003. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Alpha-Internexin (INA; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. The protein was originally purified from rat optic nerve and spinal cord. And the protein copurifies with other neurofilament subunits, as it was originally discovered, however in some mature neurons it can be the only neurofilament expressed. The protein is present in developing neuroblasts and in the Central Nervous System of adults. Meanwhile, the protein is a major component of the intermediate filament network in small interneurons and cerebellar granule cells, where it is present in the parallel fibers.

Synonyms

Alpha-internexin;Alpha-Inx;66 kDa neurofilament protein;NF-66;Neurofilament-66;Neurofilament 5;INA;NEF5;

Gene Name

Alpha-internexin

UniProt

Q16352

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Alpha Internexin recombinant protein (Position: A71-R161). Human Alpha Internexin shares 98.9% amino acid (aa) sequence identity with both mouse and rat Alpha Internexin.

Clonality

Polyclonal

Tissue Specificity

Found predominantly in adult CNS.

Applications

IF,IHC,ICC,WB,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=PB10003

Product MSDS

https://www.bosterbio.com/msds?sku=PB10003

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Immunofluorescence, 5 μg/ml, Mouse, Rat<br> Flow Cytometry(Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br>

Subcellular Location

Nucleus . Chromosome . Cytoplasm . Secreted . In basal state predominantly nuclear. .

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Protein Name

Alpha-internexin

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