CheKine™ Mirco NAD Malic Enzyme (NAD-ME) Activity Assay Kit

Malic enzyme (ME) is widely present in the cytoplasm of microorganisms, cultured cells, animals, and plants, especially with high activity in plant tissues. ME catalyzes the reversible oxidation decarboxylation of malic acid, producing pyruvate and CO2, as well as the reduction reaction accompanied by NAD (P) +, which is a key enzyme in malic acid metabolism. ME activity is closely related to biosynthesis and antioxidant activity. Based on the specificity of coenzyme and substrate, ME can be divided into NAD-ME (EC1.1.1.38) and NADP-ME (EC1.1.1.40) . NAD-ME can catalyze the reduction of NAD+ to NADH, and the rate of NADH increase at 340 nm can reflect its activity.

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Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.