Sheep anti Mouse Bub1 beta-IN

Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.

X1163P antibody detects Bub1b in mitotic NIH/3T3 cells, seperated by immunoprecipitation. Westen blot analysis (1-10 µg/mL) detects a 130 kDa band. Optimal antibody concentration should be evaluated by serial dilutions.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. Myrie, K.A., et al. Mutation and expression analysis of Human Bub1 and Bub1b in aneuploid breast cancer cell lines. Cancer Lett. 2000, 152, 193-199._x000D_ 2. Davenport, J.W., et al. The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner. Genomics 1999, 55, 113-117.