Anti-RNF25 Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-RNF25 Antibody Picoband® catalog # A10595-3. Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

E3 ubiquitin-protein ligase RNF25 is an enzyme that in humans is encoded by the RNF25 gene. The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.

Synonyms

70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Gene Name

Serine/threonine-protein kinase AtPK1/AtPK6

Gene ID

64320

UniProt

Q96BH1

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross reactivity with other proteins.

Immunogen

E.coli-derived human RNF25 recombinant protein (Position: E44-R344). Human RNF25 shares 83.7% amino acid (aa) sequence identity with mouse RNF25.

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues.

Applications

ELISA,Flow Cytometry,IF,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Asamitsu, K., Tetsuka, T., Kanazawa, S., Okamoto, T. RING finger protein AO7 supports NF-kappa-B-mediated transcription by interacting with the transactivation domain of the p65 subunit. J. Biol. Chem. 278: 26879-26887, 2003. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 9/18/2014. 3. Lorick, K. L., Jensen, J. P., Fang, S., Ong, A. M., Hatakeyama, S., Weissman, A. M. RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Proc. Nat. Acad. Sci. 96: 11364-11369, 1999.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

52588 MW

Observed Molecular Weight

62 kDa

Fragment

IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry, 2-5 μg/ml, Human, Rat Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -