Anti-MSLNL Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-MSLNL Antibody Picoband® catalog # A17693. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Loss of gene function is implicated in the emergence of novel phenotypes during organism evolution. Human MSLNL has a nonsense mutation in exon 10 and two polymorphic mutations: a frameshift in exon 3 and a nonsense codon in exon 8. The gorilla gene also shows multiple deleterious mutations, including a premature stop codon, a deletion, and a splice site mutation. Molecular evolutionary analysis indicated relaxed selection pressure on MSLNL in African great ape lineages, which suggested that MSLNL might have become inactivated before the divergence of human, chimpanzee and gorilla. The mouse Mslnl gene is highly expressed in olfactory epithelium and moderately expressed in several other tissues. We propose that the loss of MSLNL may be associated with the evolution of the olfactory system in African great apes including human.

Synonyms

Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1; PYROXD1

Gene Name

pyridine nucleotide-disulphide oxidoreductase domain 1

Gene ID

401827

UniProt

Q96KJ4

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MSLNL recombinant protein (Position: Q65-H423).

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Involved in cellular response to oxidative stress.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. B, D. W. K. A. , C, S. J. , A, D. S. K. , D, H. S. K. , A, S. B. S. , & A, Y. H. . (2012). Inactivation of the mslnl gene encoding mesothelin-like protein during african great ape evolution. Gene, 496( 1), 17-21. 2. Hollevoet, K. , Nackaerts, K. , Gosselin, R. , Wever, W. D. , & Meerbeeck, J. P. V. . (2011). Soluble mesothelin, megakaryocyte potentiating factor, and osteopontin as markers of patient response and outcome in mesothelioma. Journal of Thoracic Oncology, 6(11), 1930-1937. 3. Daniels, R. J. , Peden, J. F. , Lloyd, C. , Horsley, S. W. , & Higgs, D. R. . (2001). Sequence, structure and pathology of the fully annotated terminal 2 mb of the short arm of human chromosome 16. Human Molecular Genetics, 10(4), 339-352.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

24145 MW

Observed Molecular Weight

75 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry, 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -