Anti-NUP155 Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-NUP155 Antibody Picoband® catalog # A06391-2. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Nucleoporin 155 (Nup155) is a protein that in humans is encoded by the NUP155 gene. Nucleoporins are proteins that play an important role in the assembly and functioning of the nuclear pore complex (NPC) which regulates the movement of macromolecules across the nuclear envelope (NE). The protein encoded by this gene plays a role in the fusion of NE vesicles and formation of the double membrane NE. The protein may also be involved in cardiac physiology and may be associated with the pathogenesis of atrial fibrillation. Alternative splicing results in multiple transcript variants of this gene. A pseudogene associated with this gene is located on chromosome 6.

Synonyms

Rho-related GTP-binding protein Rho6;Rho family GTPase 1;Rnd1;RND1;RHO6;

Gene Name

Rho-related GTP-binding protein Rho6

Gene ID

9631

UniProt

O75694

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NUP155 recombinant protein (Position: D133-K715).

Clonality

Polyclonal

Tissue Specificity

Mostly expressed in brain and liver.

Applications

ELISA,IF,ICC,WB,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Gorlich, D., Mattaj, I. W. Nucleocytoplasmic transport. Science 271: 1513-1518, 1996. 2. Hawryluk-Gara, L. A., Shibuya, E. K., Wozniak, R. W. Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex. Molec. Biol. Cell 16: 2382-2394, 2005. 3. Mitchell, J. M., Mansfeld, J., Capitanio, J., Kutay, U., Wozniak, R. W. Pom121 links two essential subcomplexes of the nuclear pore complex core to the membrane. J. Cell Biol. 191: 505-521, 2010.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

26056 MW

Observed Molecular Weight

140-150 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -