Anti-Sam68/KHDRBS1 Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-Sam68/KHDRBS1 Antibody Picoband® catalog # A01717-1. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

KH domain-containing, RNA-binding, signal transduction-associated protein 1 is a protein that in humans is encoded by the KHDRBS1 gene. This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants.

Synonyms

Interferon gamma receptor 1;IFN-gamma receptor 1;IFN-gamma-R1;CDw119;CD119;IFNGR1;

Gene Name

Interferon gamma receptor 1

Gene ID

10657

UniProt

Q07666

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Sam68/KHDRBS1 recombinant protein (Position: M21-E239).

Clonality

Polyclonal

Tissue Specificity

Liver and small intestine. Also found in ovary, testis and kidney.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Associates with IFNGR2 to form a receptor for the cytokine interferon gamma (IFNG) (PubMed:7615558, PubMed:2971451, PubMed:7617032, PubMed:10986460). Ligand binding stimulates activation of the JAK/STAT signaling pathway (PubMed:7673114). .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Barlat, I., Maurier, F., Duchesne, M., Guitard, E., Tocque, B., Schweighoffer, F. A role for Sam68 in cell cycle progression antagonized by a spliced variant within the KH domain. J. Biol. Chem. 272: 3129-3132, 1997. 2. Bianchi, E., Barbagallo, F., Valeri, C., Geremia, R., Salustri, A., De Felici, M., Sette, C. Ablation of the Sam68 gene impairs female fertility and gonadotropin-dependent follicle development. Hum. Molec. Genet. 19: 4886-4894, 2010. 3. Carlosama, C., Patino, L. C., Beau, I., Morel, A., Delemer, B., Young, J., Binart, N., Laissue, P. A novel mutation in KHDRBS1 in a patient affected by primary ovarian insufficiency. (Letter) Clin. Endocr. 89: 245-246, 2018.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

54405 MW

Observed Molecular Weight

68 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse ELISA, 0.1-0.5 μg/ml, -