Anti-PRRC1 Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-PRRC1 Antibody Picoband® catalog # A15083-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

PRRC1 (Proline-Rich Coiled-Coil 1) is a Protein Coding gene. The PRRC1 gene, located on 5q23.2, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. The PRCC1 gene has two distinct regions: a proline-rich region on the N-terminus, and the DUF84 region on the C-terminus. PRCC1 is the commonly identified protein name of CAD38605. It encodes for 445 amino acids for a predicted total of 6 exons. The predicted molecular weight is 46.7 kDa, and the isoelectric point is 5.46. Orthologs have been determined in most eukaryotes, the most highly conserved being found in most mammalian species. PRRC1 is widely expressed in the thyroid, endometrium, and other tissues. Diseases associated with PRRC1 include Dystonia 23.

Synonyms

Transmembrane protein 240;TMEM240;C1orf70;

Gene Name

Transmembrane protein 240

Gene ID

133619

UniProt

Q96M27

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PRRC1 recombinant protein (Position: Y139-D411).

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Kamakari, S. , Roussou, A. , Jefferson, A. , Ragoussis, I. , & Anagnou, N. P. . (2005). Structural analysis and expression profile of a novel gene on chromosome 5q23 encoding a golgi-associated protein with six splice variants, and involved within the 5q deletion of a ph(-) cml patient. Leukemia Research, 29(1), 17-31. 2. WW Domains Provide a Platform for the Assembly of Multiprotein Networks.[J]. Molecular & Cellular Biology, 2005. 3. St-Denis, N. , Gupta, G. D. , Lin, Z. Y. , Gonzalez-Badillo, B. , Pelletier, L. , & Gingras, A. C. . (2015). Myotubularin-related proteins 3 and 4 interact with polo-like kinase 1 and centrosomal protein of 55 kda to ensure proper abscission. Molecular & Cellular Proteomics.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

19908 MW

Observed Molecular Weight

47 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -