Anti-PGK2 Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-PGK2 Antibody Picoband® catalog # A08946-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.

Synonyms

DnaJ homolog subfamily A member 2; Cell cycle progression restoration gene 3 protein; Dnj3; Dj3; HIRA-interacting protein 4; Renal carcinoma antigen NY-REN-14; DNAJA2; CPR3; HIRIP4

Gene Name

DnaJ heat shock protein family (Hsp40) member A2

Gene ID

5232

UniProt

P07205

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PGK2 recombinant protein (Position: K267-K350).

Clonality

Polyclonal

Tissue Specificity

Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Co-chaperone of Hsc70. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Chen, S.-H., Donahue, R. P., Scott, C. R. Characterization of phosphoglycerate kinase for human spermatozoa. Fertil. Steril. 27: 699-701, 1976. 2. Eicher, E. M., Cherry, M., Flaherty, L. Autosomal phosphoglycerate kinase linked to mouse major histocompatibility complex. Molec. Gen. Genet. 158: 225-228, 1978. 3. Gartler, S., Riley, D., Eddy, R., Shows, T. A human autosomal phosphoglycerate kinase gene has been assigned to chromosome 19. (Abstract) Cytogenet. Cell Genet. 40: 635-636, 1985.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

45 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -