Anti-PPAN Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-PPAN Antibody Picoband® catalog # A10836-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Suppressor of SWI4 1 homolog is a protein that in humans is encoded by the PPAN gene. The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11), an adjacent downstream gene that encodes an ATP receptor, has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation.

Synonyms

RNA-binding protein Nova-2; Astrocytic NOVA1-like RNA-binding protein; Neuro-oncological ventral antigen 2; NOVA2; ANOVA; NOVA3

Gene Name

NOVA alternative splicing regulator 2

Gene ID

56342

UniProt

Q9NQ55

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PPAN recombinant protein (Position: R20-E288).

Clonality

Polyclonal

Tissue Specificity

Brain. Expression restricted to astrocytes.

Applications

ELISA,Flow Cytometry,IF,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

May regulate RNA splicing or metabolism in a specific subset of developing neurons. Binds single strand RNA.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Communi, D., Suarez-Huerta, N., Dussossoy, D., Savi, P, Boeynaems, J.-M. Cotranscription and intergenic splicing of human P2Y11 and SSF1 genes. J. Biol. Chem. 276: 16561-16566, 2001. 2. Suarez-Huerta, N., Boeynaems, J.-M., Communi, D. Cloning, genomic organization, and tissue distribution of human Ssf-1. Biochem. Biophys. Res. Commun. 275: 37-42, 2000. 3. Welch, P. J., Marcusson, E. G., Li, Q.-X., Beger, C., Kruger, M., Zhou, C., Leavitt, M., Wong-Staal, F., Barber, J. R. Identification and validation of a gene involved in anchorage-independent cell growth control using a library of randomized hairpin ribozymes. Genomics 66: 274-283, 2000.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

29370 MW

Observed Molecular Weight

65 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -