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Anti-NRBF2 Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-NRBF2 Antibody Picoband® catalog # A09144-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Nuclear receptor-binding factor 2 is a protein that in humans is encoded by the NRBF2 gene. Involved in autophagy. Located in cytoplasm. Colocalizes with phosphatidylinositol 3-kinase complex, class III.

Synonyms

Tudor domain-containing protein 3; TDRD3

Gene Name

tudor domain containing 3

Gene ID

29982

UniProt

Q96F24

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NRBF2 recombinant protein (Position: M1-N287).

Clonality

Polyclonal

Tissue Specificity

Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Darabi, H., McCue, K., Beesley, J., Michailidou, K., Nord, S., Kar, S., Humphreys, K., Thompson, D., Ghoussaini, M., Bolla, M. K., Dennis, J., Wang, Q., and 119 others. Polymorphisms in a putative enhancer at the 10q21.2 breast cancer risk locus regulate NRBF2 expression. Am. J. Hum. Genet. 97: 22-34, 2015. 2. Flores, A. M., Li, L., Aneskievich, B. J. Isolation and functional analysis of a keratinocyte-derived, ligand-regulated nuclear receptor comodulator. J. Invest. Derm. 123: 1092-1101, 2004. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 7/22/2015.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

38 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/ml/1x10^6 cells, Human<br> ELISA, 0.1-0.5 μg/ml/ml, Human<br>

Subcellular Location

Nucleus. Cytoplasm.

Frequently Asked Questions

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