Anti-GOLGA3 Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-GOLGA3 Antibody Picoband® catalog # A07522-2. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Golgin subfamily A member 3 is a protein that in humans is encoded by the GOLGA3 gene. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins which are localized to the Golgi. Its encoded protein has been postulated to play a role in nuclear transport and Golgi apparatus localization. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

Synonyms

Formin-1;Limb deformity protein homolog;FMN1;FMN, LD;

Gene Name

Formin-1

Gene ID

2802

UniProt

Q08378

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human GOLGA3 recombinant protein (Position: Q176-K1436).

Clonality

Polyclonal

Tissue Specificity

Expressed ubiquitously.

Applications

WB,IHC,ICC,IF,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Plays a role in the formation of adherens junction and the polymerization of linear actin cables. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Bray, J. D., Chennathukuzhi, V. M., Hecht, N. B. Identification and characterization of cDNAs encoding four novel proteins that interact with translin associated factor-X. Genomics 79: 799-808, 2002. 2. Fritzler, M. J., Hamel, J. C., Ochs, R. L., Chan, E. K. L. Molecular characterization of two human autoantigens: unique cDNAs encoding 95- and 160-kD proteins of a putative family in the Golgi complex. J. Exp. Med. 178: 49-62, 1993. 3. Hicks, S. W., Machamer, C. E. The NH(2)-terminal domain of golgin-160 contains both Golgi and nuclear targeting information. J. Biol. Chem. 277: 35833-35839, 2002.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

157578 MW

Observed Molecular Weight

180 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Nucleus . Cytoplasm . Cell junction, adherens junction . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Localization to the adherens junctions is alpha-catenin-dependent. Also localizes to F-actin bundles originating from adherens junctions and to microtubules (By similarity). .