Anti-DMC1 Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-DMC1 Antibody Picoband® catalog # A02978-3. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Meiotic recombination protein DMC1/LIM15 homolog is a protein that in humans is encoded by the DMC1 gene. This gene encodes a member of the superfamily of recombinases (also called DNA strand-exchange proteins). Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. This protein, which is evolutionarily conserved, is reported to be essential for meiotic homologous recombination and may thus play an important role in generating diversity of genetic information. Alternative splicing results in multiple transcript variants.

Synonyms

Aldo-keto reductase family 1 member B10;1.1.1.-;ARL-1;Aldose reductase-like;Aldose reductase-related protein;ARP;hARP;Small intestine reductase;SI reductase;AKR1B10;AKR1B11;

Gene Name

Aldo-keto reductase family 1 member B10

Gene ID

11144

UniProt

Q14565

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human DMC1 recombinant protein (Position: D36-D283).

Clonality

Polyclonal

Tissue Specificity

Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Cloud, V., Chan, Y.-L., Grubb, J., Budke, B., Bishop, D. K. Rad51 is an accessory factor for Dmc1-mediated joint molecule formation during meiosis. Science 337: 1222-1225, 2012. 2. Habu, T., Taki, T., West, A., Nishimune, Y., Morita, T. The mouse and human homologs of DMC1, the yeast meiosis-specific homologous recombination gene, have a common unique form of exon-skipped transcript in meiosis. Nucleic Acids Res. 24: 470-477, 1996. 3. He, W.-B., Tu, C.-F., Liu, Q., Meng, L.-L., Yuan, S.-M., Luo, A.-X., He, F.-S., Shen, J., Li, W., Du, J., Zhong, C.-G., Lu, G.-X., Lin, G., Fan, L.-Q., Tan, Y.-Q. DMC1 mutation that causes human non-obstructive azoospermia and premature ovarian insufficiency identified by whole-exome sequencing. J. Med. Genet. 55: 198-204, 2018.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

36020 MW

Observed Molecular Weight

37 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -