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Anti-BEX3 Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-BEX3 Antibody Picoband® catalog # A09675-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Protein BEX3 is a protein that in humans is encoded by the NGFRAP1 gene. NGFRAP1 (nerve growth factor receptor associated protein 1), also called BEX3 (brain-expressed X-linked protein 3), NADE (p75NTR-associated death executor) or FAP-1 (Fas-associated phosphatase-1), is a 24 kDa member of the BEX protein family. A nuclear export signal (aa 77-87) allows NGFRAP1 to export bound p75NTR. It is widely expressed, but highest in ovarian granulosa cells, testis, prostate, seminal vesicle and liver. Except for a 13-19 amino acid (aa) rodent Arg/His region at aa 35 that is not found in humans, human NGFRAP1 shares 94% aa identity with mouse and rat NGFRAP1. In humans, a 101 aa isoform with an alternative start site at aa 11 has been described.

Synonyms

One cut domain family member 2; Hepatocyte nuclear factor 6-beta; HNF-6-beta; One cut homeobox 2; Transcription factor ONECUT-2; OC-2; ONECUT2; HNF6B

Gene Name

one cut homeobox 2

Gene ID

27018

UniProt

Q00994

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human BEX3 recombinant protein (Position: Q39-H103).

Clonality

Polyclonal

Tissue Specificity

Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Mukai, J., Hachiya, T., Shoji-Hoshino, S., Kimura, M. T., Nadano, D., Suvanto, P., Hanaoka, T., Li, Y., Irie, S., Greene, L. A., Sato, T.-A. NADE, a p75NTR-associated cell death executor, is involved in signal transduction mediated by the common neurotrophin receptor p75NTR. J. Biol. Chem. 275: 17566-17570, 2000. 2. Rapp, G., Freudenstein, J., Klaudiny, J., Mucha, J., Wempe, F., Zimmer, M., Scheit, K. H. Characterization of three abundant mRNAs from human ovarian granulosa cells. DNA Cell Biol. 9: 479-485, 1990. 3. Scott, A. F. Personal Communication. Baltimore, Md. 10/8/2001.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=A09675-2

Product MSDS

https://www.bosterbio.com/msds?sku=A09675-2

Observed Molecular Weight

28 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Nucleus.

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