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Anti-SRI Antibody Picoband®, Cy3 Conjugate

Selected Variant

A00222-2-Cy3

Size: 100 µg/Vial

Boster Bio Anti-SRI Antibody Picoband® catalog # A00222-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Sorcin is a protein that in humans is encoded by the SRI gene. It is mapped to 7q21.1. This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene.

Synonyms

Sorcin; 22 kDa protein; CP-22; CP22; V19; SRI

Gene Name

sorcin

Gene ID

6717

UniProt

P30626

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human SRI recombinant protein (Position: D121-A161).

Clonality

Polyclonal

Tissue Specificity

Detected in cardiac myocytes.

Applications

ELISA,Flow Cytometry,IF,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Calcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. "Entrez Gene: SRI sorcin". 2. Meyers, M B; Pickel V M; Sheu S S; Sharma V K; Scotto K W; Fishman G I (Nov 1995). "Association of sorcin with the cardiac ryanodine receptor". J. Biol. Chem. (UNITED STATES) 270 (44): 26411–8. 3. Van der Bliek AM, Baas F, Van der Velde-Koerts T, Biedler JL, Meyers MB, Ozols RF, Hamilton TC, Joenje H, Borst P (Nov 1988). "Genes amplified and overexpressed in human multidrug-resistant cell lines". Cancer Res48 (21): 5927–32.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

22 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Cytoplasm. Sarcoplasmic reticulum membrane. Peripheral membrane protein. Cytoplasmic side.

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