Anti-EDC4 Antibody Picoband®, Cy3 Conjugate

Enhancer of mRNA-decapping protein 4 is a protein that in humans is encoded by the EDC4 gene. Enhancer of mRNA decapping 4 (EDC4) was originally identified as the autoantigen Ge-1 from a Sjgren's syndrome patient later diagnosed with primary biliary cirrhosis. EDC4 (also known as HEDLS) was later identified as an essential component of cytoplasmic P-bodies responsible for mRNA decapping and degradation. Identified EDC4 protein is found as a pair of isoforms generated by alternative splicing and contains several WD domains and a putative nuclear localization signal. EDC4 co-localizes with other P-body decapping proteins such as DCP1A, DCP2 and GW182. Experimental evidence suggests that EDC4 may be involved in miRNA-mediated translation repression.

Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.

1. Bloch, D. B., Rabkina, D., Quertermous, T., Bloch, K. D. The immunoreactive region in a novel autoantigen contains a nuclear localization sequence. Clin. Immun. Immunopath. 72: 380-389, 1994. 2. Fenger-Gron, M., Fillman, C., Norrild, B., Lykke-Andersen, J. Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping. Molec. Cell 20: 905-915, 2005. 3. Garcia-Lozano, J. R., Gonzalez-Escribano, M. F., Wichmann, I., Nunez-Roldan, A. Cytoplasmic detection of a novel protein containing a nuclear localization sequence by human autoantibodies. Clin. Exp. Immun. 107: 501-506, 1997.

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Western blot, 0.25-0.5 μg/ml, Human<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>