Anti-Cbx8 Antibody Picoband® (monoclonal, 8G7), Cy3 Conjugate

Boster Bio Anti-Cbx8 Antibody Picoband® (monoclonal, 8G7) catalog # M05234. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

CBX8 functions as a transcriptional repressor and has a role in DNA damage response. This gene is mapped to chromosome 17q25.3 based on an alignment of the CBX8 sequence with the genomic sequence (GRCh38).

Synonyms

5'-AMP-activated protein kinase subunit beta-2; AMPK subunit beta-2; PRKAB2

Gene Name

protein kinase, AMP-activated, beta 2 non-catalytic subunit

Gene ID

57332

UniProt

Q9HC52

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Cbx8 recombinant protein (Position: M1-R389).

Clonality

Monoclonal

Clone

Clone: 8G7

Tissue Specificity

Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.

Applications

WB,IHC,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by inly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

References & Citations

1. Bardos, J. I., Saurin, A. J., Tissot, C., Duprez, E., Freemont, P. S. HPC3 is a new human Polycomb orthologue that interacts and associates with RING1 and Bmi1 and has transcriptional repression properties. J. Biol. Chem. 275: 28785-28792, 2000. 2. Oza, J., Ganguly, B., Kulkarni, A., Ginjala, V., Yao, M., Ganesan, S. A novel role of chromodomain protein CBX8 in DNA damage response. J. Biol. Chem. 291: 22881-22893, 2016. Note: Erratum: J. Biol. Chem. 292: 761 only, 2017.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.