Anti-MCM4 Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-MCM4 Antibody Picoband® catalog # A02301-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

MCM4 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 4), also called CDC21, S. POMBE, HOMOLOG OF, is a protein that in humans is encoded by the MCM4 gene. MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM4 gene is mapped to 8q11.21. The 864-amino acid MCM4 protein has an observed molecular mass of 97 kD by SDS-PAGE, which is similar to its calculated molecular mass of 96.6 kD. Western blot analysis with and without phosphatase treatment suggested that MCM4 is highly phosphorylated in mitotic cells. In the absence of DDK, CDK phosphorylation at the distal part of the Mcm4 NSD becomes crucial.MCM4 encodes a subunit of the MCM2-7 complex (also known as MCM2-MCM7), the replication licensing factor and presumptive replicative helicase.

Synonyms

Tumor necrosis factor receptor superfamily member 14;Herpes virus entry mediator A;Herpesvirus entry mediator A;HveA;Tumor necrosis factor receptor-like 2;TR2;CD270;TNFRSF14;HVEA, HVEM;UNQ329/PRO509;

Gene Name

Tumor necrosis factor receptor superfamily member 14

Gene ID

4173

UniProt

P33991

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MCM4 recombinant protein (Position: M361-A677).

Clonality

Polyclonal

Tissue Specificity

Widely expressed, with the highest expression in lung, spleen and thymus.

Applications

ELISA,Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. .

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

References & Citations

1. Connelly, M. A., Zhang, H., Kieleczawa, J., Anderson, C. W. The promoters for human DNA-PK(cs) (PRKDC) and MCM4: divergently transcribed genes located at chromosome 8 band q11. Genomics 47: 71-83, 1998. 2. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 3. Hu B, Burkhart R, Schulte D et al (1994). "The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins". Nucleic Acids Res. 21 (23): 5289–93.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30392 MW

Observed Molecular Weight

97 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -