Anti-LIM1/LHX1 Antibody Picoband®, Cy3 Conjugate

Boster Bio Anti-LIM1/LHX1 Antibody Picoband® catalog # A05816-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

LIM homeobox 1 is a protein that in humans is encoded by the LHX1 gene. It is mapped to 17q12. This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract.

Synonyms

LIM/homeobox protein Lhx1; LIM homeobox protein 1; Homeobox protein Lim-1; hLim-1; LHX1; LIM-1; LIM1

Gene Name

LIM homeobox 1

Gene ID

3975

UniProt

P48742

Host

Rabbit

Reactivity

Human,Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human LIM1/LHX1 recombinant protein (Position: M1-S317).

Clonality

Polyclonal

Tissue Specificity

Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Bozzi, F., Bertuzzi, S., Strina, D., Giannetto, C., Vezzoni, P., Villa, A. The exon-intron structure of human LHX1 gene. Biochem. Biophys. Res. Commun. 229: 494-497, 1996. 2. Dong, W. F., Heng, H. H., Lowsky, R., Xu, Y., DeCoteau, J. F., Shi, X. M., Tsui, L. C., Minden, M. D. Cloning, expression, and chromosomal localization to 11p12-13 of a human LIM/HOMEOBOX gene, hLim-1. DNA Cell Biol. 16: 671-678, 1997. 3. Dorus, S., Vallender, E. J., Evans, P. D., Anderson, J. R., Gilbert, S. L., Mahowald, M., Wyckoff, G. J., Malcom, C. M., Lahn, B. T. Accelerated evolution of nervous system genes in the origin of Homo sapiens. Cell 119: 1027-1040, 2004.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

45672 MW

Observed Molecular Weight

43 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.25-0.5μg/ml, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -