Anti-PLD2 Picoband® Antibody, Cy3 Conjugate

Boster Bio Anti-PLD2 Picoband® Antibody catalog # A02586. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Phospholipase D2 is an enzyme that in humans is encoded by the PLD2 gene. It is mapped to 17p13.2. The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Phospholipase D2; PLD 2; hPLD2; Choline phosphatase 2; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2; PLD2

Gene Name

phospholipase D2

Gene ID

5338

UniProt

O14939

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PLD2 recombinant protein (Position: A37-E924).

Clonality

Polyclonal

Tissue Specificity

Widely expressed.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May have a role in signal-induced cytoskeletal regulation and/or endocytosis.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Liscovitch, M., Czarny, M., Fiucci, G., Tang, X. Phospholipase D: molecular and cell biology of a novel gene family. Biochem. J. 345: 401-415, 2000. 2. Lopez, I., Arnold, R. S., Lambeth, J. D. Cloning and initial characterization of a human phospholipase D2 (hPLD2): ADP-ribosylation factor regulated hPLD2. J. Biol. Chem. 273: 12846-12852, 1998. 3. Park, S. H., Ryu, S. H., Suh, P. G., Kim, H. Assignment of human PLD2 to chromosome band 17p13.1 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 82: 225 only, 1998.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

106 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -