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Anti-IL32 Antibody (monoclonal, 2I2), Cy3 Conjugate

Boster Bio Anti-IL32 Antibody Picoband® (monoclonal, 2I2) catalog # M03286. Tested in ELISA applications. This antibody reacts with Human.

Product Specifications

Background

Interleukin 32 (Il32) is a protein that in humans is encoded by the IL32 gene. This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Synonyms

Interleukin-32; IL-32; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor; IL32; NK4; TAIF

Gene Name

interleukin 32

Gene ID

9235

UniProt

P24001

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human IL32 recombinant protein (Position: A31-K234).

Clonality

Monoclonal

Clone

Clone: 2I2

Tissue Specificity

Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.

Applications

ELISA

Purification

Immunogen affinity purified.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

References & Citations

1. Kim SH, Han SY, Azam T, Yoon DY, Dinarello CA (January 2005). "Interleukin-32: a cytokine and inducer of TNFalpha". Immunity. 22 (1): 131–42. 2. Mabilleau G, Sabokbar A (2009). "Interleukin-32 promotes osteoclast differentiation but not osteoclast activation". PLoS ONE. 4 (1): e4173.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=M03286

Product MSDS

https://www.bosterbio.com/msds?sku=M03286

Observed Molecular Weight

130 kDa

Fragment

Mouse IgG2b

Specificity

No cross reactivity with other proteins.

Applications Notes

ELISA (Cap), 1-5μg/ml, Human<br>

Subcellular Location

Secreted

Protein Name

High mobility group protein B3

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