Anti-Calbindin/CALB1 Antibody Picoband® (Biotine Conjugate)

Boster Bio Anti-Calbindin/CALB1 Antibody Picoband® catalog # PB9045. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.

Synonyms

Calbindin;Calbindin D28;D-28K;Vitamin D-dependent calcium-binding protein, avian-type;CALB1;CAB27;

Gene Name

Calbindin

UniProt

P05937

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Calbindin recombinant protein (Position: A2-E175). Human Calbindin shares 99% amino acid (aa) sequence identity with both mouse and rat Calbindin.

Clonality

Polyclonal

Tissue Specificity

Expressed in all cell types so far analyzed.

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Buffers cytosolic calcium. May stimulate a membrane Ca (2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat<br>

Subcellular Location

Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Localizes within both nucleus and cytoplasm of proliferative primary myoblasts and mostly within the nucleus of differentiated primary myoblasts. By virtue of the N-terminal PH domain, is recruited to sites of the plasma membrane containing increased PI (3,4,5)P3 or PI (3,4)P2, cell membrane targeting is also facilitared by interaction with CLIP3.