Anti-RIAM/APBB1IP Antibody Picoband® (Biotine Conjugate)

APBB1IP (APBB1-Interacting Protein), also called RIAM or RARP1, is a protein that in humans is encoded by the APBB1IP gene. By genomic sequence analysis, Lafuente et al. (2004) mapped the RIAM gene to chromosome 10p12.1. Using promoter-<a href="https://www.bosterbio.com/cells/reporter-cell-lines" style="color:#ea8d28">reporter gene</a> assays, Inagaki et al. (2003) found that RARP1 suppressed transcription from AP1 and SRE sites, but not CRE sites, in all cell lines examined. The proline-rich regions of RARP1 suppressed AP1 transactivation. Lafuente et al. (2004) found that RIAM interacted with profilin and VASP, molecules that regulate actin dynamics, as well as with RAP1-GTP.

Amyloid beta A4 precursor protein-binding family B member 1-interacting protein;APBB1-interacting protein 1;Proline-rich EVH1 ligand 1;PREL-1;Proline-rich protein 73;Rap1-GTP-interacting adapter molecule;RIAM;Retinoic acid-responsive proline-rich protein 1;RARP-1;APBB1IP;PREL1, RARP1, RIAM;

A synthetic peptide corresponding to a sequence at the C-terminus of human APBB1IP, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin- induced promoter activities through AP1 and SRE. Mediates Rap1- induced adhesion. .

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat<br> Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat<br> Immunocytochemistry , 0.5-1μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human<br>

Cell membrane ; Peripheral membrane protein . Cell projection, lamellipodium . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Colocalizes with ENA/VASP proteins at lamellipodia tips and focal adhesions, and F- actin at the leading edge. At the membrane surface, associates, via the PH domain, preferentially with the inositol phosphates, PtdIns (5)P and PtdIns (3)P. This binding appears to be necessary for the efficient interaction of the RA domain to Ras-GTPases (By similarity). .