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Anti-RCC1 Antibody Picoband® (Biotine Conjugate)

Selected Variant

A02719-1-Biotin

Size: 100 µg/Vial

Boster Bio Anti-RCC1 Antibody Picoband® catalog # A02719-1. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.

Synonyms

Short transient receptor potential channel 5; TrpC5; Transient receptor protein 5; TRP-5; hTRP-5; hTRP5; TRPC5; TRP5

Gene Name

transient receptor potential cation channel subfamily C member 5

UniProt

P18754

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RCC1 recombinant protein (Position: A14-S421).

Clonality

Polyclonal

Tissue Specificity

Expressed in brain with higher levels in fetal brain. Found in cerebellum and occipital pole.

Applications

WB,IHC,IHC-F,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective. May also be activated by intracellular calcium store depletion.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 4μg/ml, Human<br> Immunofluorescence, 4μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5μg/ml, -<br>

Subcellular Location

Cell membrane. Multi-pass membrane protein.

Price

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