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Anti-Choline Acetyltransferase/Chat Antibody Picoband® (Biotine Conjugate)

Boster Bio Anti-Choline Acetyltransferase/Chat Antibody Picoband® catalog # A01192-5. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Choline acetyltransferase  (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.

Synonyms

Choline O-acetyltransferase (EC:2.3.1.6); CHOACTase; ChAT; Choline acetylase; Chat

Gene Name

choline O-acetyltransferase

UniProt

P32738

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived rat Choline Acetyltransferase/Chat recombinant protein (Position: E19-D612).

Clonality

Polyclonal

Applications

ELISA,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=A01192-5

Product MSDS

https://www.bosterbio.com/msds?sku=A01192-5

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat<br> ELISA, 0.1-0.5μg/ml,<br>

Subcellular Location

Cytoplasm. Neuron projection.

Protein Name

Choline O-acetyltransferase

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