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Anti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5H11) (Biotine Conjugate)

Selected Variant

M03816-1-Biotin

Size: 100 µg/Vial

Boster Bio Anti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5H11) catalog # M03816-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Ubiquitin-like 1-activating enzyme E1B (UBLE1B) also known as SUMO-activating enzyme subunit 2 (SAE2) is an enzyme that in humans is encoded by the UBA2 gene. Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins

Synonyms

SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2; UBLE1B; HRIHFB2115

Gene Name

ubiquitin like modifier activating enzyme 2

UniProt

Q9UBT2

Host

Mouse

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human SAE2/UBA2 recombinant protein (Position: E449-K564).

Clonality

Monoclonal

Clone

Clone: 5H11

Applications

Flow Cytometry,WB

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Mouse IgG2b

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Human<br>

Subcellular Location

Nucleus. Cytoplasm.

Price

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