Basal Medium Eagle (BME)

Basal Medium Eagle (BME) is a basal medium used for the growth of HeLa cells, mouse fibroblasts, and other human lines, such as WI-38 and MRC-5. Supplementation is required, most commonly with 10% fetal bovine serum (FBS) for cell culture use, and requires a 5-10% CO₂ environment to maintain physiological pH. BME is modified as follows: With &bull; L-Glutamine &bull; Low Glucose &bull; Sodium Bicarbonate (2.2 g/L)&nbsp; &bull; Phenol Red Without&nbsp; &bull; HEPES &bull; Sodium Pyruvate <em>Special media modification is available as a Custom Media Service, please contact a technical representative at technical@abmgood.com for more details.</em>