DMEM/F12 Medium

DMEM/F12, or Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12), is basal medium widely used for the growth of many mammalian cells, such as MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. A 1:1 mixture of DMEM and Ham's F-12. Supplementation is required, most commonly with 10% fetal bovine serum (FBS) for cell culture use, and requires a 5-10% CO<sub>2</sub> environment to maintain physiological pH. DMEM/F12 is modified as follows: With • L-Glutamine • Sodium Bicarbonate (2.438 g/L) • Phenol Red Without  • HEPES <em>Special media modification is available as a Custom Media Service, please contact a technical representative at technical@abmgood.com for more details.</em>