Ultra-Pure BlasTaq™ 2X PCR MasterMix

Ultra-Pure BlasTaq™ 2X PCR MasterMix is a ready-to-use MasterMix containing abm’s Ultra-Pure BlasTaq™ DNA Polymerase in a uniquely-formulated buffer with gel loading dye. This strategically-engineered, next generation Taq Polymerase provides rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. BlasTaqTM has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaqTM can be used with TA cloning vectors. The enzyme is subject to a rigorous multi-step purification protocol using physical, chemical, and enzymatic methods for maximum removal of contaminating genomic DNA. Quality control involves a PCR DNA Contamination Test with three different primer sets against host DNA.