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Recombinant Human RNASE3 Protein (His Tag)

Product Specifications

Background

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

Abbreviation

RNASE3

Synonyms

RAF; RNS; Ribonuclease; RNASE3; ECP; RAF1; RNS3; Eosinophil cationic protein; Ribonuclease 3; Rnase 3; Ribonuclease A family member 3

UniProt

P12724

Accession Number

P12724

Expression System

Mammalian

Tag

C-His

Sequence

Met1-lle160

Field of Research

Cancer; Cell Biology; Immunology; Microbiology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Product Datasheet

https://789.bio/ea/rhmyyh

Product MSDS

https://789.bio/eb/SOhDO4

Calculated Molecular Weight

17.5 kDa

Observed Molecular Weight

25-30 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human

Available Sizes

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