Recombinant Human RNASE3 Protein (His Tag)
Product Specifications
Background
Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
Abbreviation
RNASE3
Synonyms
RAF; RNS; Ribonuclease; RNASE3; ECP; RAF1; RNS3; Eosinophil cationic protein; Ribonuclease 3; Rnase 3; Ribonuclease A family member 3
UniProt
P12724
Accession Number
P12724
Expression System
Mammalian
Tag
C-His
Sequence
Met1-lle160
Field of Research
Cancer; Cell Biology; Immunology; Microbiology
Endotoxin
< 1.0 EU/mg of the protein as determined by the LAL method
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
17.5 kDa
Observed Molecular Weight
25-30 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Human
Available Sizes
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