Integrin α 4 β 7 : MAdCAM-1 [Biotinylated] Inhibitor Screening ELISA Kit

Integrin alpha 4 beta 7 is an integrin family adhesion receptor that shares subunits with alpha 4 beta 1 (VLA4) and the E-Cadherin receptor, alpha E beta 7 . Integrin alpha 4 beta 7, which is critical for homing to intestinal mucosa, is induced during T cell activation in Peyer’s patches or mesenteric lymph nodes.Its expression requires signals from local dendritic and stromal cells, including secreted retinoic acid. The HIV-1 envelope protein gp120 binds to the active form of Integrin alpha 4 beta 7, and this may or may not account for the concentration of HIV-1 virus in the gut-associated lymphoid tissue (GALT). Integrin alpha 4 beta 7 may also be involved in lymphocyte trafficking in acute intestinal graft vs. host disease (GVHD).

Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new Integrin α 4 β 7 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human MAdCAM-1 to immobilized human Integrin α 4 β 7 in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated MAdCAM-1 protein, a human Integrin α 4 β 7 protein, an anti-Integrin α 4 β 7 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.