TNF-alpha [Biotinylated] : TNFR1 Inhibitor Screening ELISA Kit

Tumor Necrosis Factor (TNF), also known as TNFα, is a kind of cytokine that can kill tumor cells directly and has no obvious cytotoxicity to normal cells. TNF is a cell signaling protein involved in systemic inflammation, and it is one of the cytokines that constitute the acute phase response. It is produced primarily by activated macrophages, but most other immune cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons in the nervous system can also produce it.

1. Unopened kit should be stored at 2°C-8°C upon receiving. 2. Find the expiration date on the outside packaging and do not use reagents past their expiration date. 3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new TNF-alpha pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human TNF-alpha to immobilized human TNFR1 in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated TNF-alpha protein, a human TNFR1 protein, an anti-TNF-alpha neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.