Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.12.1/Omicron) (MALS verified)

SARS-CoV-2 Spike Trimer, His Tag (BA.2.12.1/Omicron) (SPN-C522d) is expressed from human 293 cells (HEK293) . It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (T19I, LPP24-26del, A27S, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, S704L, N764K, D796Y, Q954H, N969K, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P) ) . The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2.12.1) . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Product Specifications

Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.1 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1 (T19I, LPP24-26del, A27S, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, S704L, N764K, D796Y, Q954H, N969K, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)

Expression Region

Val 16 - Pro 1213

Host

HEK293

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS-CoV-2

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.2.12.1/Omicron) (Cat. No. SPN-C522d) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-8 ng/mL (QC tested).

Format

Powder

Buffer

0.1 M Sodium citrate, pH5.5

Additives

Trehalose

Molecular Weight

138.1 kDa

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

50ug*1

Product Datasheet

Https://www.acrobiosystems.com/static/main/products/pdf/ds/SPN-C522d.pdf

Species

SARS-CoV-2

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

TM9SF1 (NM_006405) Human Tagged Lenti ORF Clone
RC202048L3 10 µg

TM9SF1 (NM_006405) Human Tagged Lenti ORF Clone

Ask
View Details
Pgd (NM_001081274) Mouse Recombinant Protein
PM42945M5 20 µg

Pgd (NM_001081274) Mouse Recombinant Protein

Ask
View Details
Cow Prokineticin 2 / PK2 (PROK2) Protein
abx654806-01 1 mg

Cow Prokineticin 2 / PK2 (PROK2) Protein

Ask
View Details
Cow Prokineticin 2 / PK2 (PROK2) Protein
abx654806-02 5 mg

Cow Prokineticin 2 / PK2 (PROK2) Protein

Ask
View Details
CLEC11A Rabbit pAb (APR25275N)
APR25275N-01 50 µL

CLEC11A Rabbit pAb (APR25275N)

Ask
View Details
CLEC11A Rabbit pAb (APR25275N)
APR25275N-02 100 µL

CLEC11A Rabbit pAb (APR25275N)

Ask
View Details