SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2/Omicron) (MALS verified)

SARS-CoV-2 Spike Trimer, His Tag (BA.2/Omicron) (SPN-C522b) is expressed from human 293 cells (HEK293) . The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2; GISAID clade: GRA; Nextstrain clade: 21L) . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Product Specifications

Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.0 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1 (T19I, LPP24-26del, A27S, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)

Host

HEK293

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS-CoV-2

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.2/Omicron) (Cat. No. SPN-C522b) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-6 ng/mL (QC tested).

Format

Liquid

Buffer

0.1 M Sodium citrate, pH5.5

Molecular Weight

138.0 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Dry ice

Storage Conditions

-70°C

Package Size

10mg*1

Host or Source

HEK293

Species

SARS-CoV-2

Protein ID

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

NBP3-20856AF488 0.1 mL

Rabbit Monoclonal HLA ABC Antibody (MHC-I/8366R) [Alexa Fluor 488]

Ask

View Details

RR208251L4 10 µg

Stk3 (NM_031735) Rat Tagged Lenti ORF Clone

Ask

View Details

SI107375B 1 Each

Mouse Ksr1 Pre-designed siRNA Set B

Ask

View Details

abx532169 96 Tests

Human MARVEL domain-containing protein 1 (MARVELD1) ELISA Kit

Ask

View Details

BG-MUS11851 1 Each

Mouse Peptide Yy PYY ELISA Kit

Ask

View Details