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SARS-CoV-2 Spike RBD Protein, His Tag (B.1.1.529/Omicron) (Trehalose free) (MALS verified)

Selected Variant

SPD-C522f-200ug

Size: 200 µg

SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (SPD-C522f) is expressed from human 293 cells (HEK293) .

Product Specifications

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.8 kDa. The protein migrates as 33-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1 (G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493K, G496S, Q498R, N501Y, Y505H)

Host

HEK293

Target

Spike RBD

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS-CoV-2

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522f) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 2-125 ng/mL (QC tested).

Format

Liquid

Buffer

PBS, pH7.4

Molecular Weight

26.8 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Dry ice

Storage Conditions

-70°C

Package Size

200ug*1

Host or Source

HEK293

Species

SARS-CoV-2

Protein ID

QHD43416.1 (G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493K, G496S, Q498R, N501Y, Y505H)

Price

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