Biotinylated SARS-CoV-2 Spike RBD Protein (R346K, E484K, N501Y), His,Avitag™ (MALS verified)

Biotinylated SARS-CoV-2 Spike RBD Protein (R346K, E484K, N501Y), His,Avitag (SPD-C82Em) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (R346K, E484K, N501Y)). The mutations R346K, E484K, N501Y were identified in the SARS-CoV-2 Mu variant (Pango lineage: B.1.621).

Product Specifications

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 28.2 kDa. The protein migrates as 34-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Arg 319 - Lys 537

Host

HEK 293

Target

Spike RBD

Conjugation

Biotin-labeled

Tag

C-10×His & C-Avi

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated SARS-CoV-2 Spike RBD Protein (R346K, E484K, N501Y), His,Avitag (Cat. No. SPD-C82Em) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-2 ng/mL (QC tested).