SARS-CoV-2 Spike Trimer Protein (K417N, D614G), His Tag (MALS verified)

SARS-CoV-2 S protein (K417N, D614G), His Tag (SPN-C52Hm) is expressed from human 293 cells (HEK293) . It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1) . The mutations (K417N, D614G) were identified in the SARS-CoV-2 variant (known as B.1.351 or 20C/501Y.V2) which emerged in South Africa. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Product Specifications

Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.0 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1

Expression Region

Val 16 - Pro 1213

Host

HEK293

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer , His Tag (Cat. No. SPN-C52Hm) with a linear range of 1.6-50 ng/mL (QC tested).