SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS verified)
SARS-CoV-2 S protein, His Tag, Super stable trimer (SPN-C52H7) is expressed from human 293 cells (HEK293) .
Product Specifications
Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.0 kDa. The protein migrates as 170-250 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
QHD43416.1 (R683A, R685A)
Expression Region
Val 16 - Pro 1213
Host
HEK293
Target
Spike protein
Conjugation
Unconjugated
Tag
C-10xHis
Source
SARS-CoV-2
Stability
● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H7) with a linear range of 0.2-6 ng/mL (QC tested).
Format
Liquid
Buffer
0.1 M Sodium citrate, pH5.5
Molecular Weight
138.0 kDa
Shipping Conditions
Dry ice
Storage Conditions
-70°C
Package Size
1mg*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/SPN-C52H7.pdf
Species
SARS-CoV-2
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