SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS verified)

SARS-CoV-2 S protein, His Tag, Super stable trimer (SPN-C52H7) is expressed from human 293 cells (HEK293) .

Product Specifications

Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.0 kDa. The protein migrates as 170-250 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1 (R683A, R685A)

Expression Region

Val 16 - Pro 1213

Host

HEK293

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS-CoV-2

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H7) with a linear range of 0.2-6 ng/mL (QC tested).